Bjorn Eckberg, Life Assays AB
Magnetic microparticles coupled using Sub-Micron where compared to microparticles coupled using the reference reagent. The data below shows similar analytical properties when comparing both methods (Figure 1).
Material and Methods
Unconjugated superparamagnetic particles were activated by mixing with Mix&Go sub-micron. The particles were allowed to incubate on a tube rotator for one hour. The supernatant was removed and the particles were washed with ddH2O. The particles were resuspended into the solution using a vortex and sonicated until no aggregation was observed. The washing was conducted twice and the particles were then stored at +4˚C.
The anti-canine C-reactive protein (CRP) antibodies were diluted in protein diluent and added to the Mix&Go activated super paramagnetic particles. The mixture was incubated at room temperature for 30 minutes without further mixing. After the incubation, blocking diluent, with 10% w/v BSA, was added to the mixture. The mixture was then left to incubate for another 30 minutes. Once conjugation was complete the mixture was centrifuged for 30 minutes to sediment the antibody functionalized superparamagnetic particles. The supernatant was removed and a storage buffer was added. Finally, the coupled superparamagnetic particle mixture was added to our reagent for quantification of canine CRP.
"We have used the Anteo's technology for the preparation of in-vitro diagnostic reagents. The Anteo method for immobilisation of antibodies is advantageous in many ways: it is much faster than many other methods; it is much easier than many other methods; last but not least it makes use of considerably fewer hazardous chemicals which in turn decreases the health risks and waste of hazardous chemicals."