Publications

Over the past years, there have been peer-reviewed publications describing the use of Anteo’s technology in diverse applications. The publications also highlight the utility of Anteo’s coating technology deployed across diverse material substrates.

Please note: Anteo’s reagents are sometimes referred to as only "Mix&Go" in the publications, rather than Anteo's application specific reagent or product name. The below summaries indicate the reagent or product name for clarification purposes only.

 


 

Biosensor Applications

 

Elsevier

Sensors and Actuators B: Chemical

Volume 223, February 2016, Pages 89-94
Sensors and Actuators B: Chemical

An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffold

  • Carboxyl-functionalized graphene activated with Anteo's Biosensor reagent and subsequently used in electrochemical signal amplification
  • Biosensor reagent generated a stable and oriented immobilization of specific capture antibody allowed the development of an immunosensor for the APN protein involved in glucose and lipid metabolism

 

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Elsevier

Analytical Biochemistry

Volume 456, 1 July 2014, Pages 6-13
Analytical Biochemistry

Coordination complexes as molecular glue for immobilization of antibodies on cyclic olefin copolymer surfaces

  • Anteo's Biosensor reagent was a simple to use, water-based one-step surface modification reagent
  • Biosensor-activated COC demonstrated improved performance for immunoassays (TNF-aplha, TnI and TSH) in comparison to passive absorption (Fig.9)

 

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Elsevier

Analytica Chimica Acta

Volume 959, 22 March 2017, Pages 66-73
Analytica Chimica Acta

Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized double–walled carbon nanotubes

  • Electrochemical immunosensor for simultaneous determination of IL-b1 and TNF-a cytokines
  • Anteo's Biosensor reagent enabled oriented immobilization of capture antibodies and signal amplification
  • Electrochemical immunosensor improved analytical performance compared to previous approaches and ELISA methods

 

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Luminex® Platform

 

  • Using Anteo's Multiplex Microsphere activation kit, inactivated Hepatitis A virus (HAV) could be functionally immobilised on beads (difficult to do using EDC/NHS techniques)
  • The kit enabled doubling of MFI signal in comparison to EDC/NHS immobilisation (graph)
  • Anteo's Multiplex Microsphere kit provides an easy to use alternative chemistry for the immobilisation of challenging proteins

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Magnetic Particles

 

Multifunctional Magnetic Particles for Combined Circulating Tumor Cells Isolation and Cellular Metabolism Detection

Authors

  • First published: 

 

 

  • Anteo's 200nm magnetic particle functionalised with anti-EpCAM antibody utilised to isolate circulating tumor cells in blood
  • Demonstrated high cell capturing rate in blood sample (Fig. E)
  • Demonstrated high specific binding and very low non-specific binding in cell isolation application (Fig. F)

 

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Elsevier

Biosensors and Bioelectronics

Volume 88, 15 February 2017, Pages 9-14
Biosensors and Bioelectronics

Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) - β1 in urine

  • Amperometric immunosensor for quantification of TGF-β1 (a cytokine proposed as a biomarker for patients having or at risk for renal disease)
  • Carboxyl-functionalised magnetic particles coupled using Anteo's reagent demonstrated stable and oriented immobilization of the specific antibodies

  • Particles coupled using Anteo's reagent demonstrated better specific-to-non-specific ratio in the electrochemical signal amplification versus EDC/NHSS conjugated particles (see graph).

 

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